Extensive accumulation of influenza virus NS1 protein in the nuclei causes effective viral growth in vero cells.
نویسندگان
چکیده
We previously showed that modified A/Puerto Rico/8/34 (PR8) influenza master strain had improved viral rescue and growth properties in African green monkey kidney (Vero) cell line by introducing NS gene of Vero-adapted A/England/1/53 (vaEng53). In the present study, it was found that the NS1 protein derived from vaEng53 was extensively accumulated in the nuclei than that of PR8. This accumulation was caused by 7 amino acid differences in C-terminal region of NS1 protein. These results suggest that specific accumulation of NS1 protein may contribute to efficient viral replication in Vero cells.
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ورودعنوان ژورنال:
- Microbiology and immunology
دوره 51 5 شماره
صفحات -
تاریخ انتشار 2007